Example Galleries

Deep-LASI can be employed for different single-molecule assays. In this gallery, we provide a short intro on how to get started and use Deep-LASI for the different single-molecule assays. Eample data are provided together with each show case. Please click on the different images or follow the respective links to visit the corresponding example pages.

Two-color assays

Static 2c FRET

The following example on Static 2c FRET Data describes how to analyse single-molecule FRET occurring between a donor and an acceptor, which are positioned at two fixed distances apart using alternating laser excitation (ALEX).

Dynamic 2c FRET

The following section describes how to analyse Dynamic 2c FRET Data using Deep-LASI for alternating laser excitation (ALEX).

Three-color assays

Dynamic 3c FRET

In this example, we show how to analyse Dynamic 3c FRET Data using Deep-LASI for alternating laser excitation (ALEX). As example we investigate an dynamic DNA origami structure, that is labeled with three fluorophores and coordinated changes in the three inter-dye distances.